Fig. 5
From: In Vivo Analysis of DNase I Hypersensitive Sites in the Human CFTR Gene
In vivo evaluation of the 185 + 10 kb DHS. (A–F) Chromatin extracted from transgenic mouse tissues, digested with DNase I, cleaved with BamHI, electrophoresed on a 1% agarose gel, Southern blotted, and probed with cW44 H4.0 to detect the DHS in intron 1 of the CFTR gene, at 185 + 10 kb. For each tissue, lane 1 shows DNA prepared from nuclei that had not been treated with DNase I and lanes 2–5 show DNA treated with increasing amounts of DNase I: lane 2, 15 units; lane 3, 30 units; lane 4, 60 units; lane 5, 120 units. Molecular weights were estimated with respect to a 1 kb DNA ladder (Gibco BRL).