Fig. 8
Size distribution of the low-molecular-weight AGE-peptide fraction from diabetic patients on hemodialysis. Low-molecular-weight serum fractions (45 ml) obtained from diabetic patients with end-stage renal disease on hemodialysis were subjected to gel filtration chromatography (Sephadex G-15 column, 1.5 × 110 cm). Eluted fractions were monitored for non-CML AGE (○) and CML (●) by ELISA (data from triplicate determinations), AGE fluorescence (▲), and absorbance at 280 nm (△). Elution of cytochrome c (V0), vitamin B12 (1355 Da), and cytidine (243 Da) (molecular weight markers) is indicated by arrows. (A) Chromatography monitored by AGE fluorescence and absorbency at 280 nm. (B) Chromatography with affinity-purified CML or non-CML AGE antibodies obtained using 12-week or 8-week AGE-RSA, respectively.