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Fig. 4 | Molecular Medicine

Fig. 4

From: Differences in Proteinase K Resistance and Neuronal Deposition of Abnormal Prion Proteins Characterize Bovine Spongiform Encephalopathy (BSE) and Scrapie Strains

Fig. 4

Molecular mass of proteinase K-treated PrPSc elicited by different scrapie and BSE strains or isolates. Brain homogenates (10%) from C57BL/6 (BL), VM95 (VM), and CD-1 mice infected with different mouse-adapted BSE isolates and scrapie strains were treated with 50 µg/ml proteinase K for 1 hr at 37°C and run on 20% (a) or 16% SDS-PAGE (b–d) gels. PrPSc bands were revealed by immunoblotting using a rabbit anti-peptide antibody to mouse PrP sequences (aa 95–110). In c and d, PrPSc was deglycosylated by PNGase F treatment in addition to the proteinase K cleavage.

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Molecular Medicine

ISSN: 1528-3658