Fig. 6

Specifity of NF-κB-binding activity in endothelial cells in response to TNF-α. Nuclear proteins of endothelial cells treated with TNF-α (10 ng/ml) for 30 min were incubated with ³²P-labeled NF-κB oligonucleotide in the presence or absence of unlabeled oligonucleotide containing NF-κB or HSE binding element (cold oligonucleotide). Incubation with an NF-κB antibody led to a supershifted DNA-binding complex.