Fig. 3

DOX induces MBP-controlled green fluorescent protein (GFP) expression in a dose-dependent manner. Primary rat oligodendrocytes were transfected via electroporation with a construct similar to that in Figure 1, using the MBP promoter and GFP as a reporter gene. Forty-eight hours later, various concentrations of DOX as indicated in the legend were added for 24 hr, after which the cells were observed and photographed. The green fluorescence intensity from five cells in each field and five fields from each well was measured by a fluorescent microscope with a Spot camera (see text). The experiment was repeated two times.