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Fig. 2 | Molecular Medicine

Fig. 2

From: Poly(ADP-ribose) Polymerase is a Regulator of Chemokine Production: Relevance for the Pathogenesis of Shock and Inflammation

Fig. 2

PARP inhibition by PJ-34 pretreatment (30 µM) inhibits LPS-induced MIP-1α and MIP-2 mRNA accumulation in J774 cells. Lanes 1 and 2, no stimulation; lanes 3 and 4, 10 µg/ml LPS; lanes 5 and 6, PJ-34 pretreatment 30 min before LPS. β-Actin mRNA levels were unaffected by LPS or PJ-34 treatment. MIP-1α, MIP-2, and β-actin mRNA levels were quantitated using semiquantitative RT-PCR. This figure is representative of three separate experiments.

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Molecular Medicine

ISSN: 1528-3658