Fig. 5
Effect of tyrphostin (AG490) on Stat3 activation and Bcl-xL expression in SKN-SH wild-type (W) and SKN-SH/Dox6 doxorubicin-resistant (R) human neuroblastoma cells. W and R cells were treated with AG490 (10 µM) for 15 hr. The nuclear localization of Stat3 (A) and phospho-Stat3 (B) was determined by Western blot. Expression of Bcl-xL, and Bcl-xS (C) was determined in whole cell lysates using an antibody that recognized the same epitope on both molecules. 3T3 represents protein cell extract from 3T3 cells that served as control for expression of Stat3 and Bcl-xL.