Fig. 3
HPLC chromotographs of acetaldehyde-modified erythrocycle proteins. Upper panel: Separation of the erythrocyte proteins on HPLC chromatography from the samples of the alcoholics revealed the formation of fast-eluting hemoglobin fractions that reacted with the antibodies against the acetaldehyde-derived epitopes in proteins. Lower panel: Similar sites of immunologic activity also occurred in erythrocyte proteins prepared in vitro with incubation of erythrocyte proteins and 1 mM concentration of acetaldehyde. Immunologic activity was monitored using antibodies against acetaldehyde adducts. Dashed line, immunologic activity (OD405 nm). Solid line, concentration of total protein (µg/ml).