Fig. 5
From: Myocardial Ischemic Preconditioning in Rodents Is Dependent on Poly (ADP-Ribose) Synthetase
Serum levels of IL-10, in PARS+/+ and PARS−/− sham control animals and PARS+/+ and PARS−/− animals subjected to IPC after 30 min occlusion of LAD and 24 hr reperfusion. The production of IL-10 was significantly reduced in PARS2 y2 sham control mice when compared with the response in wild-type animals (p, 0.05). Ischemic preconditioning applied to PARS1 y1 mice before the 30 min occlusion significantly reduced the production of IL-10, when compared to the response in the sham control (p, 0.05). However, ischemic preconditioning markedly enhanced the production of IL-10 in the PARS2 y2 mice (p, 0.05). Thus, there was no significant difference in the plasma IL-10 levels between the preconditioned PARS2 y2 myocardial infarction group and the PARS1 y1 nonpreconditioned group. No detectable levels of the cytokine were measured in wild-type or PARS-deficient mice without myocardial occlusion and reperfusion (data not shown). Data are shown as mean 6 SEM; n 5 7–8 determinations per experimental group.