Fig. 5
From: Role of Free Radicals and Poly(ADP-ribose) Synthetase in Intestinal Tight Junction Permeability
Immunofluorescence signal. (A) For occludin, (B) ZO-1, and (C) β-catenin. The immunofluorescence signals show the typical reticular pattern in ileum section from sham-operated iNOS WT mice. We observed a significant fragmentation of the immunofluorescence signal for occludin (A1), ZO-1 (B1), and β-catenin (C1) in the ileum from zymosan-treated iNOS WT mice. Ileum fragment from zymosan-treated iNOS KO mice demonstrates less irregular distribution of occludin (A2), ZO-1 (B2), and β-catenin (C-2). Original magnification 1,600 X. (D) Control color depty coding. ZO-1; —; iNOS, inducible nitric oxide synthase; WT, wild type; KO, mice lacking iNOS.