Fig. 1
From: Accumulation of Common Clonal T Cells in Multiple Lesions of Sarcoidosis
A representative result of a RT-PCR-SSCP analysis of an intramuscular sarcoid lesion. (A) Two samples from different, widely separated sites in the intramuscular sarcoid lesion (M, M′) and PBMCs (P) were analyzed in patient 1. PBMCs exhibit mainly smear patterns. In contrast, two separate regions of the intramuscular sarcoid lesion show distinct bands of the same mobility on the smears. (B) Vβ3, 5.2, 7 and 9 subfamily analyses of the intramuscular sarcoid lesion (M, M′) and PBMCs p ](P) in patients 2 and 3 are presented. The results of the analysis are similar to those from patient 1. (C) Two samples from widely separated sites in the normal muscle specimen (M, M′) were analyzed as a control study. Although several distinct bands are observed in most Vβ families, muscle specimens from two separate portions show a different migration pattern. RT-PCR-SSCP, reverse transcriptase-polymerase chain reaction-single-strand confirmation polymorphism; PBMCs, peripheral blood mononuclear cells.