Fig. 2
From: Accumulation of Common Clonal T Cells in Multiple Lesions of Sarcoidosis
The representative results of a RT-PCR-SSCP analysis of a LN sarcoid lesion. (A) Two samples derived from widely separated sites in the lesion (L, L′) and PBMCs (P) were analyzed in patient 6. Several distinct bands are observed in most Vβ families and most of the bands in two specimens derived from separate portions of a LN sarcoid lesion show an identical migration pattern. PBMCs exhibit mainly smear patterns. (B) Vβ9 subfamily analyses of LN sarcoid lesion (L, L′) and PBMCs (P) in patients 7–10 are presented. The results of the analysis are similar to those from patient 6. (C) Two samples from lung tissue samples from a patient with early stage lung cancer (N, N′) and a pretracheal LN sample (L) were analyzed. Lung tissue shows multiple distinct bands on the smears; whereas, the pretracheal LN node tissue exhibits mainly smear patterns. RT-PCR-SSCP, reverse transcriptase-polymerase chain reaction-single-strand confirmation polymorphism; PBMCs, peripheral blood mononuclear cells; LN, lymph node.