Fig. 3
From: Accumulation of Common Clonal T Cells in Multiple Lesions of Sarcoidosis
The results of a RT-PCR-SSCP analysis of sarcoid lesions from different organs. (A) In patient 4, two samples from widely separated sites in the intramuscular sarcoid lesion (M, M′), a TBLB sample (T) and PBMCs (P) were analyzed. In Vβ4, 6, 7, 9–11, 15, and 20, several distinct bands, which are common in the TBLB sample and two samples from separate portions of an intramuscular sarcoid lesion, are observed. The arrows indicate the position of the common bands. (B) In patient 5, two samples from widely separated sites in the intramuscular sarcoid lesion (M, M′) and two samples from those in LN sarcoid lesion (L, L′), and PBMCs (P) were analyzed. In Vβ2–4 and 9 analyses, common distinct bands are observed in samples from separate portions of the LN and intramuscular sarcoid lesions. The arrows indicate the position of common bands. RT-PCR-SSCP, reverse transcriptase-polymerase chain reaction-single-strand confirmation polymorphism; PBMCs, peripheral blood mononuclear cells; TBLB, transbronchial lung biopsy; LN, lymph node.