Fig. 4
Evidence for the role of CD23/Fc ε RII in IgE-IC induction of HIV production in U1 low cells
The cells (105/ml) were first treated with IL-4 (10 ng/ml) for 48 hr, washed and then incubated in the presence of medium alone (None), IgE (10 µg/ml) for 1 hr then anti-IgE (30, 6, or 3 µg/ml). The cells were also treated with CD23-mAb or CD 19-mAb (20 µg/ml of each) for 1 hr before the addition of anti-mouse Ig (10µg/ml) to the cultures. Cells were also pre-incubated with Fab fragments of CD23-mAb (10 µg/ml) for 1 hr, washed and then incubated with IgE (10 µg/ml) and anti-IgE (30 µg/ml). Cell supernatants were harvested following 48 hr post IL-4 and the p24 quantified. Shown are mean ± SD values of two distinct U1 cell preparations, each done in duplicates.