Fig. 5
Involvement of CD23-stimulated intracellular signaling in IgE-IC-mediated induction of HIV production in U1 low cells
(A) IL-4-treated (as in Fig. 1) cells were supplemented (105/ml) with the following: 200 µM Rp-cAMP, 100 µM Dibut-cAMP, 1 mM L–NMMA, 1 mM D-NMMA, 1 mM SNAP, 200 µM SIN-1, SOD (120 U/ml) + catalase (240 U/ml), or 10 µg/ml anti-TNFα mAb. Following 2 hr of incubation, the cells were washed and treated with IgE for 1 hr then by anti-IgE and above factors as in Fig. 4. Cell supernatants were then assayed for their p24 content. Shown are mean values (SD < 17%) from three different U1 cell preparations. (B) U1low cells were or were not treated with IL-4 for 48 hr, washed and then incubated for 48 hr with decreasing concentrations of Dibut-cAMP. A dose-dependent induction of p24 is observed. Shown are mean values from two different experiments (SD < 13%).