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Table 1 IgE-mediated induction of TNFα and p24 levels in U1 low cell supernatants

From: Role of IgE Immune Complexes in the Regulation of HIV-1 Replication and Increased Cell Death of Infected U1 Monocytes: Involvement of CD23/FcεRII-Mediated Nitric Oxide and Cyclic AMP Pathways

U1low Cells Cultured with:

TNFα (pg/ml)

HIV-p24 (pg/ml)

Nonea

5 ± 3

210 ± 42

IgE (2 µg)-Anti-IgE (10 µg)

38 ± 4

321 ± 21

IgE (10 µg)-Anti-IgE (10 µg) = (IgE-IC)

125 ± 21

619 ± 36

L-NMMA

5 ± 3

183 ± 41

L-NMMA + IgE-IC

14 ± 5

441 ± 38

D-NMMA + IgE-IC

112 ± 29

601 ± 11

L-NMMA + L-arginine + IgE-IC

65 ± 18

516 ± 71

L-NMMA + D-arginine + IgE-IC

25 ± 11

449 ± 33

Rp-cAMP

2 ± 2

NDa

Rp-cAMP + IgE-IC

45 ± 13

349 ± 10

Rp-cAMP + L-NMMA + IgE-IC

8 ± 5

170 ± 32

IgE-IC + anti-TNFα mAb

NDa

401 ± 35

  1. IL-4-treated cells (as in Fig. 1) were incubated (105/ml) with IgE for 1 hr, washed and then stimulated with anti-IgE. Some cells were supplemented with anti-TNFα (10 µg/ml), l-NMMA (1 mM), D-NMMA (1 mM), L-arginine (5 mM), D-arginine (5 mM), or/and Rp-cAMP (100 µM) 1 hr before IgE-IC treatment. Following 48 hr incubation, cell supernatants were collected and assayed for their TNFα and p24 content. Values are mean ± SD from two different cell preparations each done in duplicates.
  2. aND = not determined.
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Molecular Medicine

ISSN: 1528-3658