Fig. 5

Biological active ob protein requires an intra-molecular disulfide bond

Graph showing the average weight gain at Day 10 in percentage over the weight at Day 0. Groups of five ob/ob mice were intraperitoneal injected with saline (open bar), 3 mg/kg/day mutant ob-C167A protein (strippled bar), or 3 mg/kg/day wild-type ob protein (black bar). Change in body weight between the control animals and the animals injected with the wild-type ob protein at Day 10 was significantly different (P < 0.01) as determined by a nonparametric Wilcoxon test (unpaired; two-tailed).