Fig. 2

Comparison of the concentration dependence of the toxicity of combinations of anti-CD95 and TNF under different culture conditions

Hepatocytes from BALB/c mice were left to adhere to culture plates for 6 hr in a 400-hPa athmosphere. Then medium was substituted for by pure RPMI1640 containing 333 nM ActD and various concentrations of murine TNF-α or human TNF-β (A), 333 nM ActD and various concentrations of anti-CD95 with murine TNF-α (B), anti-CD95 in combination with 5 mM GalN or 333 nM ActD (C), or 333 nM ActD and murine TNF-α in combination with various concentrations of anti-CD95 (D). Incubations were continued at 400 hPa oxygen for 20 hr (B and D), or parallel incubations were continued at 200 and 400 hPa oxygen (A and C) for 20 hr, before cytotoxicity was determined by the measurement of LDH release. Data are means ± SD, n = 3.