Fig. 2
Representative HPLC tracing of fluorophores formed by crude bovine lens homogenate after the addition of D -dopachrome as described in Materials and Methods
The mobile phase consisted of 20 mM KH2PO4 buffer (pH 4.0) and 15% methanol. The flow rate was 1.2 ml min−1. Fluorimetric detection was at 295/345 nm and is expressed in arbitrary units. Retention time: DHI 5.4 min, DHICA 7.8 min.