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Table 2 PCR and sequence analysis of V gene rearrangements of single HRS and B-NHL tumor cells from two composite lymphomas

From: Common Germinal-Center B-Cell Origin of the Malignant Cells in Two Composite Lymphomas, Involving Classical Hodgkin’s Disease and Either Follicular Lymphoma or B-CLL

Case

Biopsy

Cell Type

V Gene

No. of Positive Samples/Total

PCR Products

Repeated Rearragements#

1

Splenic

HRS

VH

13/21

13

13

 

Hilar

 

Vκ

4/21

4

4

 

Lymph node

 

Vλ

0/10

  
  

FL

VH

8/20

8

8

   

Vκ

4/20

4

4

   

Vλ

0/10

  
 

Spleen

HRS

VH

6/15

6

6

   

Vκ

1/15

1

1

 

Mesenteric

FL

VH

6/15

6

6

 

Lymph node

 

Vκ

7/15

7

7

2

Cervical

HRS

VH

6/25

6

6

 

Lymph node

 

Vκ

0/5

  
   

Vλ

10/25

10

10

  

B-CLL+

VH

4/15

4

4

   

Vκ

0/2

  
   

Vλ

4/15

4

4

 

Controls*

     

1

T cells

 

VH

0/14

  
   

Vκ

0/14

  
 

Buffer

 

VH

0/20

  
   

Vκ

0/20

  
   

Vλ

0/6

  

2

Buffer

 

VH

0/13

  
   

Vκ

0/3

  
   

Vλ

0/13

  
  1. *Summary of controls with samples of buffer covering the sections. In addition, a total of 26 controls without added cells or buffer from the sections was analysed as further negative controls, resulting in only one positive sample with three unique VκJκ joints.
  2. #The clonal VH and Vκ genes from the distinct locations and the distinct lymphoma cells of a case are clonally related.
  3. †Each sample contained two cells.
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Molecular Medicine

ISSN: 1528-3658