Fig. 3
From: The Small GTPase Rac Suppresses Apoptosis Caused by Serum Deprivation in Fibroblasts
Analysis of cell death and cell division rates of cell lines expressing different Rac mutants. (A) SRB assay after 2 days of serum withdrawal (−S), or in the presence of serum (+S), were conducted as described in the legend to Fig. 1. (B), (C), and (D) Analysis of time-lapse video microscopy data. After induction of Rac expression, the different cell lines were deprived of serum and monitored for 52 hr by time-lapse video microscopy. (B) Live cell numbers, calculated as percent of the number of cells in each field at the beginning of the observation, (C) cumulative number of dying cells, and (D) cumulative number of mitosis. For each cell line, a field of about 150 cells was examined and images were acquired at a rate of 1 frame per 8 seconds. Each point represents cumulative data over a 2-hr period. Data are representative of two independent experiments.