Fig. 4
From: The Small GTPase Rac Suppresses Apoptosis Caused by Serum Deprivation in Fibroblasts
JNK kinase activity in mutant Rac1-expressing cell lines after serum deprivation. (A) GST-Jun phosphorylation by JNK1-immunocomplexes and (B) by total JNK complexes. Proteins (300 µg) from lysates of cells deprived of serum for 4 days, were immunoprecipitated with JNK1 antibody in (A) or affinity complexed with GST-Jun in (B). Lanes are as follows: 1, control samples; 2 and 3, two independent Rac1V12 samples; and 4, Rac1N17 samples. Numbers under each lane indicate the cpm values of 32P incorporated in each band. Data are representative of two independent experiments.