Fig. 2

β-cell mass and labeling index in GTK-transgenic and CBA control mice. (A) Male and female 3- to 5-month old mice were weighed before the pancreas was dissected and used for various experiments and the pancreas weight (mg) per gram body weight was calculated (top). Values are mean ± SEM from 16–18 mice. *p < 0.05 and **p < 0.01 when compared with corresponding value of CBA control. The pancreas weight was plotted against body weight for each individual animal (bottom). (B) β-Cell area is the percentage of insulin-positive area relative to that of total pancreas area determined at 3 months of age for CBA control or GTK-transgenic mice. The labeling index values are the percent insulin-positive cells with nuclei that have incorporated [³H]-thymidine. Values are mean ± SEM for five observations. *p < 0.05 when compared with corresponding value of CBA control.