Fig. 6

Phosphorylation of kinases by cytokines. Isolated islets were cultured in media supplemented with cytokines for the indicated time points, lysed in SDS sample buffer supplemented with protease inhibitor, and subjected to Western blot analysis for phospho-Thr202/Tyr204 ERK1/2, phospho-Thr180/Tyr182 p38 MAPK, phospho-Thr183/Tyr185 JNK, and phospho-Ser473 Akt. The filters were then stripped and blotted for the total amounts of the indicated antibodies. The amounts were quantified by densitometric scannings and the relative phosphorylation was calculated as the amount of phosphorylated protein divided by the total amount. Bars are mean ± SEM from four to eight observations. *p < 0.05 and **p < 0.01 when compared with corresponding value of CBA control islets and #p < 0.05, ##p < 0.01, and ###p < 0.001 when compared with corresponding value in the absence of cytokines within each group.