Table 2 Expression of mutated protoporphyrinogen oxidase in E. coli and COS cells
Cell | cDNA | Plasmid | Total Activity nmol/g prot (mean ± SEM1) | PPOX Activity (%) |
|---|---|---|---|---|
E. coli | pUC18 | 5.7 ± 1.0 | 0 | |
Human PPOX | pUC18 | 46.8 ± 3.9 | 100 | |
78insC | pUC18 | 4.6 ± 1.4 | 0 | |
IVS2-2 a→c | pUC18 | 5.5 ± 1.3 | 0 | |
338G→C | pUC18 | 8.0 ± 1.8 | 5 | |
454C→T | pUC18 | 7.9 ± 1.2 | 5 | |
470A→C | pUC18 | 6.3 ± 1.3 | 1 | |
COS-1 | - | pCMV5 | 3.5 ± 0.5 | 0 |
Human PPOX | pCMV5 | 9.5 ± 1.0 | 100 | |
78insC | pCMV5 | 1.7 ± 0.8 | 0 | |
IVS2-2 a→c | pCMV5 | 1.7 ± 0.6 | 0 | |
338G→C | pCMV5 | 3.1 ± 0.8 | 0 | |
454C→T | pCMV5 | 3.9 ± 0.6 | 5 | |
470A→C | pCMV5 | 3.0 ± 1.5 | 0 |