Fig. 10
From: Transcriptional Regulation of the Human iNOS Gene by IL-1β in Endothelial Cells
Effect of overexpression of members of the C/EBP family of transcription factors on the transcriptional activity of the human iNOS promoter in PVEC. Cells were cotransfected with the phiNOS-205Luc plasmid, defined in Figure 8, and an expression plasmid for C/EBPα, C/EBPβ or C/EBPδ. Control cultures (first set of bars) received equal amounts of expression plasmids but without the cDNA of C/EBPα, C/EBPβ or C/EBPδ. Open bars represent the expression of luciferase reporter gene in untreated cells; closed bars represent effect of IL-1β treatment. Luciferase activity is expressed relative to that achieved in untreated PVEC cotransfected with the phiNOS-205Luc plasmid and an empty expression vector. Luciferase activity was normalized to protein concentration of cell extracts. Values shown are the means ± SEM of three independent experiments.