Fig. 11
From: Transcriptional Regulation of the Human iNOS Gene by IL-1β in Endothelial Cells
Western blot analysis of C/EBP proteins in PVEC. (A) Lanes 2 and 3 represent whole cell extracts from untreated PVEC and PVEC treated with IL-1β for 24 hr, respectively. Lane 1 represents C/EBPβ-GST fusion protein expressed in bacteria and lane 4 represents whole cell extract from COS-1 cells transfected with C/EBPβ expression plasmid. Filter was stained with antibody against C/EBPβ (sc-7962), and then stripped and stained for a second time with an antibody against β-tubulin to control for variability in loading. Molecular size markers (in kDa) are shown on the left. (B) Whole cell extracts obtained in two independent experiments from untreated PVEC (lane 1 and 4), PVEC treated with IL-1β for 24 hr (lane 2 and 5), and COS-1 cells transfected with C/EBPδ expression plasmid (lane 3 and 6) were stained with antibody against C/EBPδ (sc-151). Filter was then stripped and stained for a second time with antibody against α-tubulin to control for variability in loading. Molecular size markers (in kDa) are shown on the left.