Fig. 9
From: Transcriptional Regulation of the Human iNOS Gene by IL-1β in Endothelial Cells
Effect of overexpression of LAP (liver-enriched transcriptional activator protein) or LIP (liver-enriched transcriptional inhibitory protein) on the transcriptional activity of the human iNOS promoter in PVEC. Cells were cotransfected with the phiNOS-205Luc plasmid, defined in Figure 8, and an expression plasmid for LAP or LIP isoforms. Control cultures (first set of bars) received equal amounts of expression plasmids but without the cDNA of the LAP or LIP isoforms. Open bars represent the expression of luciferase reporter gene in untreated cells; closed bars represent effect of IL-1β treatment. Luciferase activity is expressed relative to that achieved in untreated PVEC cotransfected with the phiNOS-205Luc plasmid and an empty expression vector. Luciferase activity was normalized to protein concentration of cell extracts. Values shown are the means ± SEM of four independent experiments.