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Table 1 C/EBP sites contained within the −205/+88 bp of the human iNOS promoter and oligonucleotides used in electromobility shift assays

From: Transcriptional Regulation of the Human iNOS Gene by IL-1β in Endothelial Cells

C/EBP Sites

  

Consensus

TTNNGNAAT

 

G

G

 

−104

−96

Site #1

5′-TTTGGAAAC-3′

 

−127

−119

Site #2

5′-GTACACAAG-3′

 

−137

−129

Site #3

5 ′-TTAGCTAAC-3′

 

−191

−183

Site #4

5 ′-TGATGTAAC-3 ′

C/EBP Oligonucleotides

Consensus

5′-TGCAGATTGCGCAAT CTGCA-3′

Mutant

5′TGCAGAGACTAGTCT CTGCA-3′

C/EBP4

5′GCGACAGAG TGATGTAAC AGCAAG-3′