Fig. 1

Time course and intensity of the B. malayi γ-GT-specific antibody isotype responses in the sera of BALB/c mice immunized with aqueous or Alum-precipitated B. malayi γ-GT. Mice (n = 6) from each group were injected i.p. with 10 µg recombinant γ-GT or γ-GT/AL on days 0, 15, and 28 and i.v. on days 35 and 61. Individual sera obtained prior to the immunizations were tested for the presence of γ-GT-specific antibodies by class- and subclass-specific ELISA. Bars represent the geometric mean value ± SD of 6 individuals, γ-GT-specific Igs’ concentrations are either in µg/ml or ng/ml. Figure 1A, IgG1; Figure 1B IgG2a; Figure 1C. IgG3; Figure 1D. IgA. Similar results were observed in 3 repeated experiments. They all were significantly different from the saline-immunized group since no specific antibodies were detected in this group.