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Fig. 7 | Molecular Medicine

Fig. 7

From: Lipopolysaccharide Signals Activation of Tumor Necrosis Factor Biosynthesis Through the Ras/Raf-1/MEK/MAPK Pathway

Fig. 7

CMV promoter activity and CRE promoter activity are not influenced by cotransfection with dominant inhibitors of ras or raf-1

The CMV promoter, which is expressed constitutively in RAW 264.7 cells, and a CRE-containing promoter, which is expressed in RAW 264.7 cells in response to dibutyryl cAMP stimulation, were used to drive CAT mRNA transcription and cotransfected into RAW 264.7 cells together with empty vector (control) or constructs encoding dominant ras or raf-1 inhibitors. CAT activity was assayed 48 hr after transfection and 24 hr after stimulation with 8-bromo-cAMP (in the case of the CRE promoter-CAT system). Little or no inhibitory effect resulted from inhibitor cotransfection.

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