Fig. 5

Inhibition ELISA carried out with recombinant proteins representing each allelic form of PvMSP1₁₉. ELISA plates were coated with a bacterial recombinant protein representing PvMSP1₁₉ Belém allele. (A) The indicated recombinant fusion protein or GST was added at different concentrations in each well. Human serum from individual 40 was added at a dilution of 1:800. Results are expressed as the average of triplicate samples ± SD. (B) Average inhibition of the binding of human sera to recombinant protein PvMSP1₁₉ Belém. The indicated soluble recombinant proteins or GST was added to a final concentration of 10 µg/ml. The percentage of inhibition was calculated as described in Materials and Methods, individuals were selected for group I when inhibition values with either recombinant protein were >90%. Group II represents individuals who had inhibition of <90% when the recombinant protein PvMSP1₁₉ Asia was used. Results are expressed as the average of the indicated number of individuals ± SD.