Fig. 1
From: ATP Controls Neuronal Apoptosis Triggered by Microtubule Breakdown or Potassium Deprivation
Time course of caspase activation by microtubule disrupting agents. CGC were incubated with nocodazol (1 µM) or colchicine (1 µM). At the time points indicated, the percentage of apoptotic nuclei was counted in cultures stained with H-33342 (A). Enzymatic caspase-activity (DEVD-afc cleavage) (B) and processing of procaspase-3 (C) were determined in parallel cultures. Recombinant caspase-3 (20 ng/lane) was used as positive control for Western blotting. Data are means ± SEM from seven determinations. Error bars are smaller than the data symbols.