Fig. 2
From: ATP Controls Neuronal Apoptosis Triggered by Microtubule Breakdown or Potassium Deprivation
Prevention of colchicine-induced caspase activation by taxol. CGC were treated with colchicine (1 µM) in the presence or absence of taxol (1 µM) for 18 hr. Parallel cultures were used for quantitating the percentage of apoptotic neurons (condensed chromatin) for measuring caspase activity [DEVD-afc cleavage in pmol/(min × mg)] and for immunocytochemistry (staining of βIII-tubulin). Nuclei were stained with H-33342 and correspond to the same field used for imaging the tubulin structure. The images were obtained by confocal microscopy (×63, NA 1.4 lens), and the width of every panel corresponds to 100 µm.