Fig. 7

Apoptosis prevention and translational block: a comparison of CHX and DG effects. (A) CGC were exposed to colchicine for 18 hr in the presence or absence of CHX (1 µM) or ActD (150 nM) until caspase activity (pmol/(min × mg) was determined enzymatically and tubulin structure was examined by immunostaining. (B) Different inhibitors (2 mM DG, 1 µM CHX, 100 µM zVAD-fmk, 150 nM ActD) were added to CGC cultures either 1 hr before colchicine (−1) or at different times after colchicine as indicated on the ordinate. All experiments were stopped 18 hr after colchicine addition. Data are presented as percent of the apoptosis, observed with colchicine alone. Experiments were performed 3–4 times and averages of all experimental data are shown. (C) CGC were incubated with different concentrations of CHX or DG at 8 hr after exposure to colchicine (1 µM). Inhibition of translation by DG or CHX was measured in the period of 90–180 min after their addition; inhibition of apoptosis was measured 9 hr later. The different inhibitor concentrations are indicated in the figure. Data are averages of three experiments.