Confocal microscopy of the MitoTracker Red-related fluorescence in N-HFFs versus DS-HFFs. Representative images show that the mitochondrial network is less fragmented in (a) N-HFFs than in (b) DS-HFFs. c The number of mitochondria, measured using the Fiji software (http://www.fiji.sc), is significantly higher in trisomic cells compared with non-trisomic cells. (D) The average mitochondrial volume is significantly lower in DS cells compared with non-trisomic cells. The bars show mean values ± SEM of two non-trisomic and two trisomic cell cultures. Fifteen randomly selected cells for each sample/experimental condition were analyzed. **p ≤ 0.05
Cells were obtained from the ‘Telethon Bank of Foetal Biological Samples’ at the University of Naples. All experimental protocols were approved by the local Institutional Ethics Committee. Abbreviations: N-HFFs: Euploid human fetal fibroblasts; DS-HFFs: Trisomic human fetal fibroblasts; a.u.: arbitrary units.