Fig. 5From: Stachydrine protects eNOS uncoupling and ameliorates endothelial dysfunction induced by homocysteineSTA increased the levels of GTPCH1 and DHFR. a Relative mRNA levels of GTPCH1 and DHFR were assessed by using qRT-PCR. b Protein levels were assessed by using Western blotting. c Quantification of GTPCH1 and DHFR protein levels, which was normalized to the β-actin levels. d The mRNA levels of GTPCH1 and DHFR were assessed by using qRT-PCR. e GTPCH1 and DHFR protein levels were assessed by using Western blotting. f Quantification of GTPCH1 and DHFR protein levels as in (e). g BAECs were incubated with 10 μM STA for indicated time periods, GTPCH1 and DHFR protein levels were assessed by using Western blotting. h Quantification of GTPCH1 and DHFR protein levels as in (g). Data shown were mean ± SEM, n = 5. *P < 0.05Back to article page