Fig. 1
From: Non-invasive fetal sex diagnosis in plasma of early weeks pregnants using droplet digital PCR
Experimental strategy. a Isolation of circulating DNA (maternal and fetal) from plasma obtained from pregant women at different gestational ages and analysis of fetal sex by qPCR and ddPCR. b An automated system blends oil samples into a cartridge and generates droplets of nanolitre size by a microfluidic system and vacuum generation. c The emulsion is transferred to the thermocycler for the amplification. The presence of the target template in the droplets will be detected by the fluorescence development. d Detection of fluorescence using the Droplet Reader. The system, after the injection of a reader oil, separates and aligns each droplet, which is analyzed by two lasers to detect fluorescence. e Analysis of the data represented in graphs as the fluorescence (FAM or HEX) relative to each droplet (Event Number)