Fig. 3
From: Non-invasive fetal sex diagnosis in plasma of early weeks pregnants using droplet digital PCR
Representative graphs for circulating DNA samples at different gestational ages analyzed by ddPCR. Three circulating DNA samples, extracted from maternal plasma at different gestational weeks and carrying male (a, #21: 7th week; #29: b, 4,5th week) or female fetus (c, #23:7th week), were analyzed in ddPCR for SRY (FAM) and EIF2C1 (HEX) gene targets. For all three samples, the upper graphs correlate the FAM fluorescence intensity, corresponding to the SRY gene (blue dots), to the number of positive events; the lower graphs report the HEX fluorescence intensity, relative to the EIF2C1 reference gene (green dots), to the number of positive events. Black dots indicate negative events (no amplification events). The threshold lines are coloured in fuchsia