Fig. 3

Effect of preimmunization with αDCIR2-HA_109–117 on Thy1.1⁺ and Foxp3⁺ cells (HA_109–117 abbreviated in the figure as HA due to space concerns). Naïve TCR transgenic CD4 T cells recognizing HA_109–117 peptide were injected into immunocompetent congenic recipients, which were subsequently injected with either αDEC-205-HA_109–117 or αDCIR2-HA_109–117 fusion mAb. In both, a cases antigen-specific Thy1.1+ T cells can be tracked in various peripheral lymphoid organs, and c αDCIR2 immunization results in somewhat increased proliferation. b However, on day 14, essentially all Thy1.1⁺ T cells appeared to be deleted in mice immunized with αDCIR2-HA, whereas significant populations of Thy1.1⁺ T cells could still be detected in mice that had received the same amount of αDEC-205-HA_109–117 fusion mAb. Additionally, immunization with αDEC-205-HA_109–117 mAb results in increased Foxp3 expression, as detected on d day 7 and e day 14. In contrast, immunization with αDCIR2-HA_109–117 mAb leads to a marginal increase in Foxp3 expression on d day 7, and e efficient deletion of all cells on day 14