Table 1 The primer sequences of mRNA
Gene | Amplification sequences of mRNA |
|---|---|
cAMP | F: 5’-GCTAACCTCTACCGCCTCCT-3’ |
R: 5’-GGTCACTGTCCCCATACACC-3’ | |
PKA | F: 5’-CAGGAAAGCGCTCCAGATAC-3’ |
R: 5’-AAGGGAAGGTTGGCGTTACT-3’ | |
CREB | F: 5’-TACAGGATAGACTAGCCACTT-3′ |
R: 5’-AATATGTTTTCCTATCGGGGT-3’ | |
β-actin | F: 5’-GGGCACAGTGTGGGTGAC-3’ |
R: 5’-CTGGCACCACACCTTCTAC-3’ |