Fig. 2From: Assessment of HIF-1α expression and release following endothelial injury in-vitro and in-vivoWestern blot analysis of HIF-1α. a Different concentrations of HIF-1α standard protein and b cell lysates from the cells cultured in 21% or 1% O2. HIF-1α standards and in cell lysates showed a band at a molecular weight of approximately 130 kDa. GAPDH (loading control) showed a band at a molecular weight of approximately 36 kDa. Numbers on the left indicate the migration of molecular weight standardsBack to article page