Fig. 1
From: B-1a cells protect mice from sepsis-induced acute lung injury
Adoptive transfer of B-1a cells attenuates lung inflammation. a Peritoneal washout cells isolated from healthy mice were stained with anti-mouse Pacific Blue-CD23, FITC-B220 and PE-Cy5 Abs and subjected to sort purification by using a flow cytometry-based cell sorting system. A total of 5 × 105 B-1a cells suspended in 150 μl of PBS were delivered into the peritoneal cavity of CLP mice. After 20 h, lung tissue was harvested and mRNA and protein expression of b, c IL-6, d, e IL-1β, f, g TNF-α, h, i IFNγ and j, k IL-10 were assessed, respectively. Data are expressed as means ± SE (n = 9 mice/group) and compared by one-way ANOVA and SNK method (*p < 0.05 vs. sham mice; #p < 0.05 vs. PBS-treated CLP mice). CLP, cecal ligation and puncture; IL, interleukin