Fig. 4

Treatment with B-1a cells attenuates apoptosis in lungs after sepsis. After 20 h of CLP, lung tissues were collected from PBS or B-1a cell treated mice. a Lung tissue sections were prepared for TUNEL staining shown in green, and for nuclear staining using PI shown in red. Representative images at × 100 original magnification are shown. Scale bar, 100 μm. b TUNEL positive apoptotic cells were counted at 18 random fields in a blinded fashion, and the average numbers of cells per field are shown. c Cleaved Caspase-3 activity in total lung tissues of sham-operated, and PBS or B-1a cell-treated CLP mice was determined. Data are expressed as means ± SE (n = 6 mice/group) and compared by one-way ANOVA and SNK method (^*p < 0.05 vs. shams; ^#p < 0.05 vs. PBS-treated CLP mice). CLP, cecal ligation and puncture; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; PI, propidium iodide