Fig. 3
From: AEG-1 is involved in hypoxia-induced autophagy and decreases chemosensitivity in T-cell lymphoma
Effect of HIF-1α on AEG-1 and autophagy markers in T-NHL cells exposed to hypoxia. HIF-1α siRNA and the negative control (Scrambled siRNA) were transfected into Hut-78 and Jurkat cells, respectively. The mRNA expression of HIF-1α in Jurkat cells (a, p < 0.05) and Hut-78 cells (b, p < 0.05) were measured with RT-PCR. c Expression of HIF-1α, AEG-1, Beclin-1, LC3-I and LC3-II in Jurkat cells were detected by western blot. d Quantitative analysis of HIF-1α (p < 0.05), AEG-1 (p < 0.05), Beclin-1 (p < 0.01) and LC3-II (p < 0.01) in Jurkat cells. e Quantitative analysis of ratio of LC3-II/LC3-I (p < 0.01) in Jurkat cells. f Expression of HIF-1α, AEG-1, Beclin-1, LC3-I and LC3-II in Hut-78 cells were detected by western blot. g Quantitative analysis of HIF-1α (p < 0.05), AEG-1 (p < 0.01), Beclin-1 (p < 0.01) and LC3-II (p < 0.01) in Hut-78 cells. h Quantitative analysis of ratio of LC3-II/LC3-I (p < 0.01) in Hut-78 cells. *p < 0.05, **p < 0.01. HIF-1α siRNA vs.Scrambled siRNA