Fig. 1

KW6002 treatment did not affect normal postnatal development of retinal vasculature. a Schematic of the experimental design: KW6002 was administered by intraperitoneal injection at volume of 10 mg/kg from P7 to P15 every other day. Mice were euthanized at P17.(Sac:Sacrifice). b Mouse whole-mount retinas from KW6002- and vehicle-treated mice were harvested and immune-stained with isolectin B4. The retinal vasculature morphologies were indistinguishable between KW6002- or vehicle-treated pups at P17 in room-air. Scale bar: 500 μm. c The retinal vasculatures of the superficial, intermediate and deep vascular layers were examined at P17 by isolectin B4 staining of whole-mount retinas. The distributions of three retinal vascular layers were displayed in distinct confocal planes. Vessel density was quantified as the ratio of the total vessel length to microscopic field. The vessel densities of the superficial, intermediate and deep plexuses were indistinguishable between KW6002-treated and vehicle-treated pups. Scale bar: 50 μm