Fig. 2

KW6002 treatment attenuated retinal avascular area and pathological angiogenesis at P17 in OIR. a Schematic of the experimental design: KW6002 was administered by intraperitoneal injection at volume of 10 mg/kg from P7 to P15 every other day. OIR mice were euthanized at P17. b Following the OIR, retinal vasculatures from KW6002- and vehicle-treated mice were visualized by whole-mount isolectin B4 at P17. Avascular areas are indicated by red dotted line. Neovascularization tufts are indicated by purple line. Scale bar: 500 μm. c Quantitative analysis of avascular areas by isolectin B4 and the neovascular tufts area showed that KW6002 treatment reduced avascular areas(Student’s t-test) and neovascular tufts area(Mann-Whitney U test)compared to the vehicle-treated pups. ***P < 0.001, n = 11–13/group. d Representative H-E staining images showing the neovascular nuclei numbers (red arrow) on the vitreal side of the inner limiting membrane. Scale bar: 20 μm. e Quantitative histochemical analysis showed that KW6002 treatment (P7–17) reduced neovascular nuclei numbers as compared to the vehicle-treated pups. ***P < 0.001, Student’s t-test, n = 11–13 /group