Fig. 5

KW6002 treatment reduced hyperoxia-induced retinal vascular regression at P9 and P12 and cellular apoptosis at P12, does not affect the activation of microglia. Whole-mount retinas (a, c) or retinal cross-section (e, g) from the vehicle-OIR and KW6002 groups were harvested on P9 (a) or P12 (c, e, g) and examined by immunostaining with isolectin B4, or histological analysis by TUNEL or Iba-1. Representative images show the avascular areas (indicated by red dotted line) at P9 (a) and P12 (c) (Scale bar: 500 μm) and TUNEL⁺ cells (indicated by yellow arrow) (e) or Iba-1⁺ cells (indicated by yellow arrow) (g) in retinal sections at P12 (Scale bar: 50 μm). Areas of vaso-obliteration (b, d) and TUNEL⁺ cells (f) or Iba-1+ cells (h) were quantified and analyzed **P < 0.01, ***P < 0.001, Student’s t-test or Mann-Whitney U testn = 8 /group