Fig. 6

H19 expression was regulated by CTCF occupancy in the H19 imprinting control region. a, b Paravertebral muscles were harvested from AIS patients during the fusion surgery. The tissues were subjected to Nuclei preparation and chromatin digestion, and the CTCF-binding status at the H19 ICR were assessed with CHIP assay, as described in Materials and Methods. a represent electrophoretogram of PCR product by 10% polyacrylamide gel electrophoresis. b CTCF occupancy in the H19 ICR showed significantly reduced occupancy in the concave-sided muscle tissue samples. The experiments were performed three times. Results are expressed as mean ± SEM. n = 4 in each group. **, P < 0.01 versus concave side