Fig. 3
From: Propofol inhibited autophagy through Ca2+/CaMKKβ/AMPK/mTOR pathway in OGD/R-induced neuron injury
Autophagy induction abolished the PPF-mediated amelioration of OGD/R-triggered cell injury. OGD/R-exposed neurons were pre-treated with the autophagy activator rapamycin (Rapa, 30 μM), followed by propofol (PPF, 30 μM in 0.1% DMSO) treatment. a, b Western blot was performed to evaluate the effects of autophagy activator rapamycin on protein expression of autophagy-related genes LC3, Beclin1, and p62. Quantification of western blots was shown. c LDH release, d CCK-8 cell viability, and e cell apoptosis assay were performed to evaluate whether autophagy was involved in the PPF-mediated amelioration of OGD/R-triggered cell injury. Values are represented as the mean ± SD from three independent experiments. *p < 0.05 vs. Control group; #p < 0.05 vs. DMSO group; $p < 0.05 vs. PPF group